Coding

Part:BBa_K2683001:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)


Arc Protein Codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 531
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for use in E.coli .

Source

The original sequence is derived from the Arc protein found in mouse neural cells. https://www.uniprot.org/uniprot/Q9WV31

References

[1]Pastuzyn, E., Day, C., Keams, R., Kyrke-Smith, M., Taibi, A., McCormick, J., Yoder, N., Belnap, D., Erlendsson, S., Morado, D., Briggs, J., Feschotte, C., and Shepard, J. (2018) The Neuronal Gene Arc encodes a repurposed retrotransposon Gag protein that mediates intercellular RNA transfer. cell. 172, 275-288